MEDIA CREATION IN TISSUE CULTURE

Abstract

Plant tissue culture is part of an unconventional vegetative propagation technique. The difference in this technique compared to conventional vegetative propagation techniques is usually located in different situations and locations. Tissue culture media is one of the factors that can determine the success rate of in vitro plant propagation. Components of culture media are environmental factors that provide elements of plant growth such as macro nutrient elements, micro nutrient elements, carbohydrates, vitamins and growth regulators, organic salts, natural complex compounds, activated charcoal and compacting materials (Various formulations or compositions of growing media It was found to optimize the growth and development of cultured plants, there are several types of media, but the culture media commonly used are media with the Murashige and Skoog (MS) formulation, MS media are basic media that have a very complete formulation.In general, tissue culture media are divided into basic media and treatment media. Recipe for basic media is a recipe for a combination of substances containing essential nutrients (macro and micro), sources of energy and vitamins.