STERILIZATION OF PLANT TISSUE CULTURE EQUIPMENT

Abstract

This practicum aims to learn the methods and principles of sterilization of tools and materials in tissue culture techniques. The function of the sterilized tools is to avoid the presence of microorganisms that are still carried by the tools that will be used, because the presence of microorganisms causes contamination and can even grow bacteria that are not completely sterile. Tissue culture tools were carried out on December 8, 2019 at the Yahdi Tissue Culture Laboratory, Jl. Ambung, Tanah Enam Ratus, Medan Marelan, Medan City, North Sumatra.The materials used in the Tissue Culture Tool Sterilization practicum include: detergent/soap and clean water. The tools used in the tissue culture tool sterilization practicum include: culture bottles. The conclusion of this practicum is that the Sterilization tools are carried out in two stages, namely Stage I Sterilization, the bottles are sterilized or cleaned with detergent and soaked for 10-15 with bleach water (pr°C lin), while in Stage II Sterilization, the tools are sterilized using an autoclave at a temperature of 1210 C for 1 hour 20 minutes.